Here is discussed the theory of retention in chromatography from a thermodynamic point of view. You also find an introduction to the concepts of adsorption isotherm and surface excess and their roles in chromatography.

 

 

In the surface properties section you find a brief summary of the chemical and physical properties of the silica surface and of reversed phase surfaces. 

 

In the mobile phase section you find a short description of the role of the mobile phase in reversed phase chromatography.

 

A discussion concerning the retention mechanism in reversed phase chromtaography is found in the retention theory section.

 

Tutorials about basic chromatographic theory and concepts is found in studyhplc.com.

 

 

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