LIQUID CHROMATOGRAPHY; SCIENCE OR ART?
When working with method development or trouble shooting of liquid chromatographic methods, one usually comes to a point where the method is not working as well as one would like it too. The action taken is often to try to solve a problem by testing more or less vague ideas. The reasoning, “Maybe the problem can be solved if I change this or that parameter?” is frequently applied in practice. It is tempting to believe that the only way to solve a problem is by trial and error or by applying some tricks obtained from a colleague or some other source.
One may ask; “Is this the most rational approach to problem solving in liquid chromatography?”.This question is important for those who work with the development of chromatographic methods.
For more than 40 years, thousands of scientists and engineers have worked on the development of HPLC. The result is one of the most versatile techniques that we have in chemical science today. Furthermore, the development is still going on with thousands of papers and many books being published every year. All this has been accomplished because there is an understanding of the basic physico-chemical principles of the chromatographic process. As an expert in liquid chromatography you will also need to understand these basic principles and to learn how they are implemented into your daily work.
Those who have high aspirations and want to become qualified experts or scientitsts in liquid chromatography now have a unique opportunity to apply to an Advanced Course in Liquid Chromatography. The course is given by Professor Jan Ståhlberg, Academy of Chromatography, and can be followed on-line. Read more about this course.
Take a closer look at this site to learn more about the fundamentals of Reversed Phase Chromatography.
What is chromatography? Click here to see an animated simulation of the chromatographic process!
In this site you find tutorial material concerning:
- Thermodynamic background to chromatography.
- Properties of reversed phase stationary phases.
- The role of the mobile phase in reversed phase chromatography.
- Discussion about the retention mechanism of reversed phase chromatography.
This tutorial is for those responsible for the HPLC equipment in a laboratory, working with trouble-shooting or develop standard HPLC-methods. For these types of work you need to have a good understanding of the different concepts and basic theory of chromatography.
Development of advanced HPLC methods requires a thorough understanding of chromatographic theory. If you are working in this area of chromatography, this site will give you a start concerning reversed phase chromatography.
Do you want to learn more about the basic concepts of chromatography? Visit studyhplc.com.
I whish you good luck with your studies in chromatography. Enjoy it, it´s interesting!
Comments or questions?
Send an e-mail to studyhplc.com
Send an e-mail to studyhplc.com
